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By Georg Gübitz, Alexander Bauer, Guenther Bochmann, Andreas Gronauer, Stefan Weiss

Michael Lebuhn, Stefan Weiß, Bernhard Munk, Georg M. Guebitz

Microbiology and Molecular Biology instruments for Biogas approach research, prognosis and Control

Veronika Dollhofer, Sabine Marie Podmirseg, Tony Martin Callaghan, Gareth Wyn Griffith & Katerina Fliegerová

Anaerobic Fungi and their capability for Biogas Production

Bianca Fröschle, Monika Heiermann, Michael Lebuhn, Ute Messelhäusser, Matthias Plöchl

Hygiene and Sanitation in Biogas Plants

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Direct Interspecies Electron move in Anaerobic Digestion: A Review

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A severe evaluation of Microbiological Biogas to Biomethane Upgrading Systems

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Influent Fractionation for Modeling non-stop Anaerobic Digestion Processes

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Fate of hint Metals in Anaerobic Digestion

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Extra resources for Advances in Biochemical Engineering Biotechnology Metabolic Engineering

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Unfortunately, the use of high copy expression plasmids to increase PKS gene copy number has not been possible, which may either be due to plasmid instability or toxicity effects. 42 R. McDaniel et al. A final consideration for overexpression of PKSs is the post-translational phosphopantethienylation required to generate biosynthetically active PKS enzymes. This modification is performed by an enzyme called a holo-ACP synthase [66] (Fig. 3). Co-expression of a holo-ACP synthase with appropriate specificity is required for expression of PKSs in E.

Temperature (often done in shake flasks), pH, dissolved oxygen tension, and agitation rate, which affects both shear and dissolved oxygen [40]. All of these variables have been shown to affect culture growth and productivity in a variety of organisms [4, 19, 40, 41]. , products synthesized after the growth phase of a fermentation, this is not universally the case. For example, novel polyketides produced by heterologous hosts may demonstrate some degree of growth-associated production. In addition, media and culture conditions can be manipulated to result in production of secondary metabolites during the exponential growth phase.

23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. Metabolic Engineering for L-Lysine Production by Corynebacterium glutamicum 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. edu Polyketide natural products are a rich source of bioactive substances that have found considerable use in human health and agriculture. Their complex structures require that they be produced via fermentation processes. This review describes the strategies and challenges used to develop practical fermentation strains and processes for polyketide production.

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