By Muhammad Sarwar Khan, Iqrar Ahmad Khan, Debmalya Barh
Applied Molecular Biotechnology: the following iteration of Genetic Engineering explains state of the art advances within the swiftly constructing sector of molecular biotechnology, the expertise of the hot millennium. produced from chapters authored through prime specialists of their respective fields, this authoritative reference text:
- Highlights the most recent omics-based instruments and techniques utilized in smooth biotechnology
- Explains how a variety of molecular biology applied sciences can be utilized to strengthen transgenic vegetation and the way these crops can meet turning out to be nutrition and plant-derived product demands
- Discusses chloroplast gene expression structures, mitochondrial omics, plant practical genomics, and whole-genome resequencing for crop improvement
- Explores plant–microbe and plant–insect interactions affecting plant safeguard and productivity
- Covers animal types, pharmacogenomics, human tissue banking, and the molecular prognosis of illnesses akin to cervical melanoma, weight problems, and diabetes
- Examines the molecular features of viral ailments, creation of business commodities utilizing viral biotechnology, and biotechnological makes use of of magnetic nanoparticles
- Describes using biotechnology within the foodstuff, chemical, pharmaceutical, environmental conservation, and renewable strength sectors
Applied Molecular Biotechnology: the subsequent new release of Genetic Engineering serves as a springboard for brand spanking new discoveries in molecular biology and its functions. hence, this e-book is a useful source for college kids and researchers of molecular biotechnology.
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Extra resources for Applied molecular biotechnology : the next generation of genetic engineering
5. Ion-exchange chromatography: Separation on the stationary phase based on the charge present on them. Proteins with a net negative charge stick on to beads possessing positive charge and vice versa. Proteins are released by counterions and eluted by gradually raising the concentration of monovalent ions or changing the pH of mobile phase. 6. Hydrophobic interaction chromatography: It separates proteins based on the hydrophobic interaction acting between the protein and the matrix coated with hydrophobic groups.
It has emerged as one of the most promising advances made since the green revolution, especially for designing crops resistant to various pests, pathogens, and environmental stresses. Since the past years, crops such as soybean, cotton, corn, canola, squash, and papaya are being grown commercially for one or the other transgenic traits. The two most popular techniques for genetic transformation are Agrobacterium tumefaciens mediated transfer and particle bombardment. With the advent of new techniques in plant transgenesis, plant transformation vectors have also improved giving high-transformation frequencies without host range limitations.
Pulse field gel electrophoresis DNA greater than ~40 kb length cannot be easily separated by applying constant electrical field. This problem is solved by pulsed field gel electrophoresis (PFGE) in which electric field is switched periodically between two different directions. Agarose gel electrophoresis It is frequently used for qualitative and quantitative estimation of nucleic acid, that is, DNA and RNA. Isoelectric focusing It separates the proteins in a pH gradient based on their isoelectric point (pI).